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9780199634255

PCR 2: A Practical Approach - M. J. McPherson - Hardcover

PCR 2: A Practical Approach - M. J. McPherson - Hardcover
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  • ISBN-13: 9780199634255
  • ISBN: 0199634254
  • Edition: 1
  • Publisher: Oxford University Press, Incorporated

AUTHOR

McPherson, M. J., Hames, B. David, Taylor, G.R.

SUMMARY

1. Optimizing PCR 2. Use of specialty phosphoramidites in PCR 3. Chemical methods for 5' non-isotopic labelling of PCR probes and primers 4. Solid-phase PCR 5. Solid-phase sequencing of PCR products 6. cDNA cloning by RT - PCR 7. Quantification of DNA and RNA by PCR 8. PCR MIMICS: competitive DNA fragments for use in quantitative PCR 9. In vitro expression of proteins from PCR products 10. PCR-based approaches to human genome mapping 11. Fingerprinting of DNA and RNA using arbitrarily primed PCR 12. Mutational analysis 13. Mutational analysis: new mutations 14. Linear amplification for the in virto study of ligand/DNA interactions 15. Ligand-mediated PCRMcPherson, M. J. is the author of 'PCR 2: A Practical Approach - M. J. McPherson - Hardcover' with ISBN 9780199634255 and ISBN 0199634254.

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