7335044
9780120443055
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The new series METHODS IN MOLECULAR GENETICS provides practical experimental procedures for use in the laboratory. Because the introduction of molecular genetic techniques has revolutionized biological research, a wide range of methods is covered. This volume of METHODS IN MOLECULAR GENETICS presents up-to-date practical molecular biology and genetics techniques of the analysis of microbial genes and chromosomes, including those od eukaryotic andf prokaryotic cells and plasmids. * * Methods presented for easy use and ready adaptation to new systems * Detailed experimental protocols included for: * * Eukaryotic microbes - yeast (mutants, transposons, viruses), parasites (gene identificationa dn regulatino), slime mold (transformation) * * Bacterial DNA and Chromosmes - codon usage, quantitation of RNA transcription, challenge phage, cell division, motility and chemotaxis * * Bacterial Gene Analysis - gel shift assay, DNase 1 footprinting, gene fusions, membrane protein genes, oxidative stress genes * * Plasmids - assays for DNA and DNA-binding proteinsAdolph, Kenneth W. is the author of 'Molecular Microbiology Techniques Part A', published 1994 under ISBN 9780120443055 and ISBN 0120443058.
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