GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression

The b-glucuronidase (GUS) gene is extremely useful as a reporter of the expression of introduced genes and can be used in organisms where other reporter genes are useless. Not only can this assay be used to detect whether a gene is being expressed, but it can be used to determine the location of the gene product within cells. GUS Protocols provides instructions and essential background information that will enable researchers to effectively use the GUS gene as a reporter of the expression of introduced genes. Key Features and Benefits * Written by leading authorities * Foreword by R.A. Jefferson, inventor of the GUS assay * Up-to-date reference lists following each of the fourteen chapters * Illustrated with nineteen full-color photographs * Offers clearly written, straightforward, easy-to-follow procedures for * Using the GUS system in both plants and animals * GUS detection by fluorometric, spectrophotometric, and histochemical methods * Fast, automated assays * Provides background information on * The b-glucuronidase reaction * Substrates * Instrumentation * Construction of GUS fusion vectorsGallagher, Sean R. is the author of 'GUS Protocols: Using the GUS Gene as a Reporter of Gene Expression', published 1992 under ISBN 9780122740107 and ISBN 0122740106.