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Publication Date: 2000
Publisher: Cold Spring Harbor Laboratory Press
Stephen T. Smale, Michael Carey
After a review of the state-of-the-art in the RNA polymerase II transcription field, subsequent chapters present a systematic guide to the investigation of regulatory mechanisms for newly identified genes, and discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering their mode of action. Carey (UCLA) and Smale (Howard Huges Medical Institute and UCLA) write for graduate students, physician-scientists, and others, either beginning or already established, who are undertaking in-depth studies in the analysis of gene expression in model systems or disease states. They put methods in the context of underlying theory, recommend experimental strategies, encourage creativity in investigative design, and explain protocols, with troubleshooting advice.Stephen T. Smale is the author of 'Transcriptional Regulation in Eukaryotes: Concepts, Strategies and Techniques', published 2000 under ISBN 9780879695378 and ISBN 0879695374.
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