2032282

9781588292339

Oligonucleotide Synthesis Methods and Applications

Oligonucleotide Synthesis Methods and Applications
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  • ISBN-13: 9781588292339
  • ISBN: 1588292339
  • Publication Date: 2004
  • Publisher: Humana Press

AUTHOR

Herdewijn, Piet

SUMMARY

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.Herdewijn, Piet is the author of 'Oligonucleotide Synthesis Methods and Applications', published 2004 under ISBN 9781588292339 and ISBN 1588292339.

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