Multiple Detection In Size-exclusion Chromatography
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  • ISBN-13: 9780841238787
  • ISBN: 0841238782
  • Publication Date:
  • Publisher: American Chemical Society



Multiple Detection in Size-Exclusion Chromatography provides acomprehensive view of detection techniques that are used synergistically insize-exclusion chromatography (SEC). These techniques include differentialrefractometry; static and dynamic light scattering; differential viscometry;fluorescence, ultraviolet, infrared, and nuclear magnetic resonancespectroscopy; electrospray ionization, matrix-assisted laser desorptionionization, chemical reaction interface, inductively coupled plasma, and tandemmass spectrometry; and dynamic surface tension. This book shows how thesetechniques provide useful molar mass, architectural, compositional, andthermodynamic information whether SEC is used as the sole separation method oras part of a two-dimensional chromatographic set-up. In the latter case, theability to separate analytes based on size, provided by SEC, is augmented byseparation based on chemical composition, block length, etc., provided by highperformance liquid chromatography, liquid chromatography at the criticalcondition, phase fluctuation chromatography, or high osmotic pressurechromatography.A large number of applications are shown throughout the book, exemplifying bothnatural and synthetic polymers. These include, but are not restricted to, thestudy of long- and short-chain branching in polyolefins; the study of methylmethacrylate polymers, copolymers, and oligomers; elemental speciation inenvironmental and biological samples; the characterization of oligo- andpolysaccharides; the analysis of complex protein mixtures and of glycoproteinsand protein-polymer conjugates; the study of polymer reaction kinetics; thedetermination of chemical and compositional heterogeneity; the characterizationof semi-flexible synthetic polymers and polypeptides; and the study ofchromatographic band broadening.Striegel, Andre M. is the author of 'Multiple Detection In Size-exclusion Chromatography', published 2004 under ISBN 9780841238787 and ISBN 0841238782.

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