Methods in Gene Technology 1991

Contents. List of Contributors. Introduction. Principles of Nucleic Acid Hybridization (J.J. McFadden). Basic Procedures for 5'- and 3'-End Labeling and Generating cDNA Probes (P.G. Sanders and J.W. Dale). Synthesis of RNA Probes Using SP6, T7 and T3 RNA Polymerase (P.A. Krieg). Labeling of Probes by the Random Primer Procedure (A.P. Feinberg). Film Detection Procedures for Radioactive Isotopes (W.M. Bonner). Non-Isotopic Labeling and Detection (G.R. Coulton and A.D.B. Malcolm). Photobiotin Labeling of DNA and RNA Hybridization Probes (J.L. McInnes and R.H. Symons). Nucleic Acid Hybridizations with Positive Charge-Modified Nylon Membrane (K.C. Reed). Plaque and Colony Screening Using Nucleic Acid Probes (K.J.H. Robson). In Situ Hybridization (A.J. Easton and R.P. Eglin). Characterization of mRNA Transcripts by S1 Mapping and Primer Extension (J.N. Hansen and S. Banerjee). The Polymerase Chain Reaction: Basic Procedures and Use for Virus Detection (J.P. Clewley). The Use of Polymerase Chain Reaction for cDNA Synthesis (T.M.J. Leu, C.C. Kuenzle, and M.W. Berchtold). RFLPs: Uses and Methods of Detection (K. Johnson and E. Nimmo). Transcription-Based Amplification System (D.Y. Kwoh, T.J. Kwoh, and T.R. Gingeras). Index.Dale, Jeremy W. is the author of 'Methods in Gene Technology 1991' with ISBN 9781559382632 and ISBN 1559382635.