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Publication Date: 1993
Publisher: Oxford University Press, Incorporated
Stern, Claudio D., Holland, Peter W.
Part I. Basic techniques: obtaining and handling embryos 1. Drosophila embryos 2. Nematode embryos 3. Embryos and larvae of invertebrate deuterostomes 4. Zebrafish embryos 5. Xenopus 6. Avian embryos 7. Post-implantation mammalian embryos 8. Simple tips for photomicrography or embryos Part II. Experimental Embryology 9. Fate maps and cell lineage analysis 10. Microinjection into Drosophila embryos 11. Cell transplantation in Xenopus 12. Transplantation in avian embryos 13. Chick limb buds 14. Nuclear transplantation 15. Laser ablation of cells Part III: Cellular techniques 16. Studying cell movements in vivo by time-lapse video microscopy 17. Induction assays in Xenopus 18. Culture of avian neural crest cells 19. ES cells and gene targeting 20. Introduction of genes using retroviral vectors Part IV: Molecular techniques 21. Immunocytochemistry of embryonic material 22. RNase protection assays 23. cDNA library construction 24. Analysis of gene expression by RT-PCR 25. Cloning genes using PCR 26. In situ hybridisation Appendices I. Stage tables. a. Westerfield for zebrafish embryos b. Nieukoop & Faber for Xenopus laevis c. Eyal-Giladi & Kochav for early chick d. Hamburger & Hamilton for later chick II: Suppliers of specialist items IndexStern, Claudio D. is the author of 'Essential Developmental Biology: A Practical Approach (Cloth)', published 1993 under ISBN 9780199634231 and ISBN 0199634238.
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