Histological+histochemical Methods

9780080362243

0080362249

Since the first edition of this book appeared, there have been many advances in histochemistry. In order to encompass new methods likely to become standard in research, this second edition has been thoroughly revised and updated to incorporate the latest developments. The organisation of the text has been altered so that theoretical and practical materials are now more closely integrated, with instructions for methods now immediately following the explanations of principles, and the theoretical exercises at the end of each chapter have been expanded. The continuing diversity of methods for fixing and processing are covered, with more prescribed methods being given than before, including the most modern developments in haematological staining, nucleic acid and carbohydrate histochemistry, immunohistochemistry and other affinity-based staining methods. New techniques in carbohydrate histochemistry are discussed, including important developments in conventional histochemistry and methods based on the use of lectins. No other book gives comparable coverage of traditional and contemporary staining methods for light microscopy, which include fixation, use of dyes, enzyme biochemistry, immunohistochemistry and special methods for blood and cell suspensions and for nervous tissue. Chemical and physical principles of each major group of methods are explained, with detailed practical instructions for selected methods, so that even those with minimal prior knowledge of chemistry will be able to follow the text.