Flow Cytometry

A flow cytometer measures physical and chemical characteristics of single cells or organelles and enables them to be identified, counted, and physically separated (sorted) for further study. Flow cytometry is little more than 20 years old and is still unfamiliar to many biologists. The rapidincrease in the number of machines being installed has meant that there are many comparatively inexperienced workers trying to cope with the new technology. This book offers practical guidance in preparing and staining their samples. It should also enable more experienced practitioners who want toinvestigate a new application.The second edition covers similar ground to the first. For example, chapters on fluorescence technology, DNA analysis and the measurement of cell proliferation, chromosome analysis and sorting, membrane potential and pH, and the further applications to cell biology, have been updated and, wherenecessary, expanded. New authors have written enlarged chapters on immunofluorescence of surface markers and measurement of cytoplasmic and nuclear antigens.Ormerod, M. G. is the author of 'Flow Cytometry' with ISBN 9780199634620 and ISBN 0199634629.