Calcium Signalling A Practical Approach
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9780199638482
ISBN:0199638489
Edition: 2nd Pub Date: 2001Publisher: Oxford University Press, Incorporated Summary: This new edition focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometric techniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear e [read more]
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9780199638482
ISBN:
0199638489
Edition: 2nd
Pub Date: 2001
Publisher: Oxford University Press, Incorporated
This new edition focuses on the applications of fluorescent and bioluminescent techniques for studying calcium signalling and calcium dependent processes. Cameleon probes, targeting bioluminescent probes, low affinity fluorescence indicators and chelators, and photometric techniques are all covered in depth. Also described in detail are methods for monitoring mitochondrial function, measuring calcium in the nuclear envelope and nucleoplasm, measuring calcium dependent gene expression, monitoring calcium signals in multicellular preparations, and measuring calcium extrusion. A major problem of fluorescent calcium measurements is the artifacts from zinc and transition metals and a chapter is devoted to their detection and minimization. Some of the pharmacological methods of studying calcium release are also covered. With many of the chapters written by the very scientists who developed the methods they are discussing this book is the most up-to-date, invaluable book for those studying cellular calcium.
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