Bioanalytical Chemistry

9780471544470

0471544477

A timely, accessible survey of the multidisciplinary field of bioanalytical chemistryBioanalytical chemistry is playing an increasingly central role in the fields of academic and industrial science. It overlaps with a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics-all of which makes a comprehensive introduction to the subject even more essential for students and researchers.Featuring many of the techniques used by biochemists and molecular biologists, Bioanalytical Chemistry reviews all relevant aspects of using analytical methodology to solve biological problems. The end result is an authoritative primer to this expanding field-one that will prove invaluable for advanced undergraduate chemistry and biochemistry students, bioanalytical chemistry graduate students, and practitioners. Each chapter in the book begins with coverage of basic concepts, then progresses to applications that involve real samples. Bioanalytical Chemistry covers: Spectroscopic methods for matrix characterization Structural and functional properties of antibodies Principles of electrophoresis Centrifugation methods Mass spectrometry of biomolecules Quantitation of enzymes and their substrates Design and implementation of enzyme assays Isoelectric focussing Chromatography of biomolecules Validation of new bioanalytical methods And much more This indispensable guide features end-of-chapter problems and selected answers in the appendix, designed to help students assess their knowledge of each topic. Each chapter also includes suggestions for further reading, and mathematical derivations have been kept to a minimum throughout.More than any other reference, Bioanalytical Chemistry captures the interdisciplinary scope of this emerging field-and uniquely prepares students for collaborative scientific work and research in industrial, government, and academic laboratories.