Live Cell Imaging

Dedication DVD Legends Preface Section 1: DETECTION AND APPROACHES TO LIVE CELL IMAGING 1. Fluorescent Protein Tracking and Detection M. Rizzo and D. Piston2. Constructing and Expressing GFP Fusion Proteins D. Spector and R. Goldman3. Viral Vectors for Introduction of GPF R. Lansford4. Gene Delivery by Direct Injection and Facilitation of Expression by Mechanical Stretch D. Dean5. Microinjection of Fluorophore–labeled Proteins Y. Komarova, J. Peloquin, and G. Borisy6. CCD Cameras for Fluorescence Imaging of Living Cells P. Tran7. Photobleaching Techniques to Study Mobility and Molecular Dynamics of Proteins in Live Cells: FRAP, iFRAP, and FLIP G. Rabut and J. Ellenberg8. FRET and Fluorescence Lifetime Imaging Microscopy M. Tramier, D. Sanvitto, V. Emiliani, C. Durieux, and M. Coppey–Moisan9. Monitoring Protein Dynamics Using FRET–based Biosensors T. Chew and R. Chisholm10. Application of Light–directed Activation of Caged Biomolecules and CALI to Problems in Cell Motility D. Humphrey, Z. Rajfur, B. Imperiali, G. Marriott, P, Roy, and K. Jacobson11. Photoactivation–based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus J. Politz, R. Ruft, and T. Pederson12. Fluorescent Speckle Microscopy of Cytoskeletal Dynamics in Living Cells T. Wittman, R. Littlefield, and C. Waterman–Storer13. Polarization Microscopy with the LC–PolScope R. Oldenbourg14. Confocal Microscopy, Deconvolution, and Structured Illumination Methods J. Murray15. Multiphoton and Multispectral Laser–scanning Microscopy M. Dickinson16. Analyzing Live Cell Data and Tracking Dynamic Movements W. Tvarusko, J. Mattes, and R. Eils SECTION 2: IMAGING OF LIVE CELLS AND ORGANISMS 17. In Vivo Imaging of Mammalian Cells J. Swedlow, P. Andrews, and M. Platani18. A Sealed Preparation for Long–term Observation of Cultured Cells G. Sluder, J. Nordberg, F. Miller, and E. Hinchcliffe19. Live Cell Imaging of Yeast D. Rines, D. Thomann, J. Dorn, P. Goodwin, and P. Sorger20. Live Imaging of Caenorhabditis elegans B. Podbilewicz and Y. Gruenbaum21. Time–lapse Cinematography in Living DrosophilaTissues I. Davis and R. Parton22. Single–cell Imaging in Animal Tumors In Vivo J. Wyckoff, J. Segall, and J. Condeelis23. Long–term, High–resolution Imaging in the Neocortex In Vivo B. Chen, J. Trachtenberg, A. Holtmaat, and K. Svoboda24. Intravital Microscopy of Normal and Diseased Tissues in the Mouse R. Jain, E. Brown, L. Munn, and D. Fukumura25. Development of Mammalian Cell Lines with lacOperator–tagged Chromosomes Y. Strukov and A. Belmont26. Imaging Gene Expression in Living Cells S. Janicki and D. Spector27. Studying Mitosis in Cultured Mammalian Cells P. Wadsworth28. Imaging Hoechst–33342–labeled Chromosomes and Fluorescent Proteins During the Cell Cycle T. Haraguchi and Y. Hiraoka29. Imaging the Actin Cytoskeleton A. Matus, V. Biou, H. Brinkhaus, and M. Roelandse30. Imaging Intermediate Filament Proteins in Living Cells E. Kuczmarski and R. Goldman31. Methods for Expressing and Analyzing GFP–Tubulin and GFP–Microtubule–associated Proteins H. Goodson and P. Wadsworth32. Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells J. Lippincott–Schwartz and E. Snapp33. Imaging Live Cells Under Mechanical Stress B. Helmke and P. Davies34. Imaging Single Molecules Using Total Internal Reflection Fluorescence Microscopy N. Stuurman and R. Vale35. Visualization and Quantification of Single RNA Molecules in Living Cells Y. Shav–Tal, S. Shenoy, and R. Singer Appendix 1: Cautions IndexSpector, David is the author of 'Live Cell Imaging ', published 2004 under ISBN 9780879696832 and ISBN 0879696834.