International Review Neuro Biology

Focussing on the vertebrate brain, the new edition of this successful book contains all of the information required for newcomers to achieve successful in situ hybridization results and will enable those already using the technique to improve their methodology. Fully updated and revised to take into account recent developments, this book provides user-friendly, clear, detailed protocols together with helpful illustrations and troubleshooting tips. Part I describes the well tried and tested technique using radioactive labelled oligonucleotides and Part II covers non-radioactive in situ hybridization methods. New chapters include: cryostat sectioning of brains; processing of retinal tissue for in situ hybridization; processing the spinal cord for in situ hybridization; processing human brain tissue for in situ hybridization; and, non-radioactive in situ hybridization: simplified procedures for use in whole mounts of mouse and chick embryos.Wisden, W. is the author of 'International Review Neuro Biology' with ISBN 9780123668479 and ISBN 0123668476.